全文获取类型
收费全文 | 447篇 |
免费 | 41篇 |
专业分类
电工技术 | 3篇 |
化学工业 | 184篇 |
金属工艺 | 3篇 |
机械仪表 | 11篇 |
建筑科学 | 22篇 |
矿业工程 | 1篇 |
能源动力 | 14篇 |
轻工业 | 84篇 |
水利工程 | 6篇 |
无线电 | 17篇 |
一般工业技术 | 84篇 |
冶金工业 | 7篇 |
原子能技术 | 2篇 |
自动化技术 | 50篇 |
出版年
2024年 | 1篇 |
2023年 | 16篇 |
2022年 | 10篇 |
2021年 | 92篇 |
2020年 | 33篇 |
2019年 | 27篇 |
2018年 | 33篇 |
2017年 | 22篇 |
2016年 | 24篇 |
2015年 | 21篇 |
2014年 | 23篇 |
2013年 | 33篇 |
2012年 | 20篇 |
2011年 | 32篇 |
2010年 | 17篇 |
2009年 | 19篇 |
2008年 | 15篇 |
2007年 | 8篇 |
2006年 | 11篇 |
2005年 | 6篇 |
2004年 | 5篇 |
2003年 | 5篇 |
2002年 | 2篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1990年 | 1篇 |
1984年 | 1篇 |
1981年 | 1篇 |
排序方式: 共有488条查询结果,搜索用时 15 毫秒
71.
Elisabetta Delibato Fabrizio Anniballi Paola Sinibaldi Vallebona Giuseppe Palleschi Giulia Volpe Marina Nadia Losio Dario De Medici 《Food Analytical Methods》2013,6(4):996-1003
Foodborne disease caused by Salmonella represents a worldwide public health problem. In Europe, salmonellosis is still the second most commonly recorded zoonosis. Since the standard culture method for detecting Salmonella (ISO 6579:2002) requires up to 5 days to produce results, the need to develop rapid methods represents an important issue for the authorities and the producers. The aim of the present study was the in-house validation, according to ISO 16140, of an open-formula diagnostic real-time PCR for the detection of Salmonella in all the different meat categories reported in the EU Regulations relative to microbiological criteria for food safety. The assay employed specific primers and a probe target within the ttrRSBCA locus, which allows the tetrathionate respiration in Salmonella. Selectivity, relative accuracy, relative sensitivity and relative specificity were established by testing 110 bacterial strains and 175 various edible meat samples. Results showed 100 % selectivity, 100 % relative accuracy, 100 % relative sensitivity and 100 % relative specificity of the real-time PCR when compared to the standard culture method used as reference. In addition, in order to minimize the effect of the competitive micro-flora naturally present on meat samples, a highly nutritious and selective commercial medium (ONE Broth Salmonella, Oxoid) was evaluated in comparison with the classical non-selective pre-enrichment broth (buffered peptone water). Results demonstrated that the ONE Broth Salmonella medium increases the growth of Salmonella in the presence of competitive micro-flora. 相似文献
72.
Enrica Omiccioli Giulia Amagliani Giorgio Brandi Mauro Magnani 《Food microbiology》2009,26(6):615-622
Intoxications and infections caused by food-borne pathogens represent an increasing public health problem, and diagnostic tests in multiplex format are needed for the rapid identification of food contaminations caused by more than one microbial species. We have developed a multiple PCR-based platform for the simultaneous detection of the widespread milk-associated pathogens Salmonella spp., Listeria monocytogenes and Escherichia coli O157. The assay combines an enrichment step in a medium properly formulated for the simultaneous growth of target pathogens, a DNA isolation method, and a multiplex Real-Time PCR detection system based either on dual-labelled probes (mRT-PCR), or on melting curve analysis (mHRM). The second, producing a distinct peak for each amplification product, allows the qualitative assessment of pathogen presence. Moreover, the internal amplification control (IAC) included in the reaction, ensuring the reliability of results, complies with quality management programmes. Inclusivity and exclusivity were 100% each, with a detection limit of 1 CFU for each pathogen in a total of five 25 ml-aliquots of raw milk, and a duration of two working days.The assay represents an alternative approach for the qualitative detection of the cited bacterial species, suitable for a relatively inexpensive screening of several milk samples, reducing the turnaround time and the workload. 相似文献
73.
74.
The strong anisotropy of unidirectionally-reinforced FRP composites makes it difficult to design an efficient anchorage for measuring the tensile strength and this difficulty increases together with the cross section area of the specimen. To overcome this difficulty a new anchor system was developed for tension testing of large diameter GFRP bars in a universal testing machine. The anchor body designed and tested consists of two shells, pushed one against the other by the jaws of the testing machine, leaving an inner conical hole. The ends of the bar are provided with resin heads having the shape of the hole inside the anchor body. The present paper details the modelling investigation to verify and improve the shape and hence the performances of this anchor system. The approach adopted consists in a parametric numerical analysis that rests on the experimental data already gathered. 相似文献
75.
Vaz Isabela C. F. Macchi Carlos Eugenio Somoza Alberto Rocha Leandro S. R. Longo Elson Cabral Luis da Silva Edison Z. Simões Alexandre Zirpoli Zonta Giulia Malagù Cesare Desimone P. Mariela Ponce Miguel Adolfo Moura Francisco 《Journal of Materials Science: Materials in Electronics》2022,33(15):11632-11649
Journal of Materials Science: Materials in Electronics - This study reports the electrical properties of Nd-doped cerium oxide (CeO2) films synthesized by microwave assisted hydrothermal using a... 相似文献
76.
Michele Serri Giuseppe Cucinotta Lorenzo Poggini Giulia Serrano Philippe Sainctavit Judyta Strychalska-Nowak Antonio Politano Francesco Bonaccorso Andrea Caneschi Robert J. Cava Roberta Sessoli Luca Ottaviano Tomasz Klimczuk Vittorio Pellegrini Matteo Mannini 《Advanced materials (Deerfield Beach, Fla.)》2020,32(24):2000566
Magnetic crystals formed by 2D layers interacting by weak van der Waals forces are currently a hot research topic. When these crystals are thinned to nanometric size, they can manifest strikingly different magnetic behavior compared to the bulk form. This can be the result of, for example, quantum electronic confinement effects, the presence of defects, or pinning of the crystallographic structure in metastable phases induced by the exfoliation process. In this work, an investigation of the magnetism of micromechanically cleaved CrCl3 flakes with thickness >10 nm is performed. These flakes are characterized by superconducting quantum interference device magnetometry, surface-sensitive X-ray magnetic circular dichroism, and spatially resolved magnetic force microscopy. The results highlight an enhancement of the CrCl3 antiferromagnetic interlayer interaction that appears to be independent of the flake size when the thickness is tens of nanometers. The estimated exchange field is 9 kOe, representing an increase of ≈900% compared to the one of the bulk crystals. This effect can be attributed to the pinning of the high-temperature monoclinic structure, as recently suggested by polarized Raman spectroscopy investigations in thin (8–35 nm) CrCl3 flakes. 相似文献
77.
78.
Giulia Morettini Claudio Braccesi Filippo Cianetti 《Fatigue & Fracture of Engineering Materials & Structures》2019,42(4):827-837
In the literature there are many experimental results of multiaxial fatigue testing, usually generated by the combination of two or more cyclical loads. The cases in which samples are randomly stressed are rarer. Moreover, to generate particular stress states, the use of specific machinery for fatigue tests is usually required. For these reasons, the authors have created a particular geometry of specimens, which, when solicited by a single input of a random type, guarantees the creation of specific multiaxial stress states without using complex and costly instruments. The experimental tests were finally used for the validation of the multiaxial reduction method developed and currently utilized in the authors' design phase, though potentially used to verify all the other methodologies present in the literature. 相似文献
79.
Nanometre-sized diamond has been found in meteorites, protoplanetary nebulae and interstellar dusts, as well as in residues of detonation and in diamond films. Remarkably, the size distribution of diamond nanoparticles seems to be peaked around 2-5 nm, and to be largely independent of preparation conditions. We have carried out ab initio calculations of the stability of nanodiamond as a function of surface hydrogen coverage and of size. We have found that at about 3 nm, and for a broad range of pressures and temperatures, particles with bare, reconstructed surfaces become thermodynamically more stable than those with hydrogenated surfaces, thus preventing the formation of larger grains. Our findings provide an explanation of the size distribution of extraterrestrial and of terrestrial nanodiamond found in ultradispersed and ultracrystalline diamond films. They also provide an atomistic structural model of these films, based on the topology and structure of 2-3-nm dimond clusters consisting of a diamond core surrounded by a fullerene-like carbon network. 相似文献
80.
Ultrastable Liquid–Liquid Interface as Viable Route for Controlled Deposition of Biodegradable Polymer Nanocapsules 下载免费PDF全文
Raffaele Vecchione Giulia Iaccarino Paolo Bianchini Roberto Marotta Francesca D'autilia Vincenzo Quagliariello Alberto Diaspro Paolo A. Netti 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(22):3005-3013
Liquid–liquid interfaces are highly dynamic and characterized by an elevated interfacial tension as compared to solid–liquid interfaces. Therefore, they are gaining an increasing interest as viable templates for ordered assembly of molecules and nanoparticles. However, liquid–liquid interfaces are more difficult to handle compared to solid–liquid interfaces; their intrinsic instability may affect the assembly process, especially in the case of multiple deposition. Indeed, some attempts have been made in the deposition of polymer multilayers at liquid–liquid interfaces, but with limited control over size and stability. This study reports on the preparation of an ultrastable liquid–liquid interface based on an O/W secondary miniemulsion and its possible use as a template for the self‐assembly of polymeric multilayer nanocapsules. Such polymer nanocapsules are made of entirely biodegradable materials, with highly controlled size—well under 200 nm—and multi‐compartment and multifunctional features enriching their field of application in drug delivery, as well as in other bionanotechnology fields. 相似文献